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high-pressure homogenizer avestin emulsiflex c3  (AVESTIN Inc)

 
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    AVESTIN Inc high-pressure homogenizer avestin emulsiflex c3
    High Pressure Homogenizer Avestin Emulsiflex C3, supplied by AVESTIN Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/emulsiflex-c3+homogenizer/emulsiflex+c5+homogenizer/pm40639378-222-5-7
    Average 90 stars, based on 1 article reviews
    high-pressure homogenizer avestin emulsiflex c3 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Application of Amber Suppression To Study the Role of Tyr M210 in Electron Transfer in Rhodobacter sphaeroides Photosynthetic Reaction Centers.
    Article Snippet: In each round, the cell slurry was passed more than three times through an Avestin EmulsiFlex-C3 Homogenizer operating at 20,000 – 25,000 psi.

    Article Title: Structural determinants for pH-dependent activation of a plant metacaspase.
    Article Snippet: Cells were lysed by using an EmulsiFlex-C3 Homogenizer (Avestin, Ottawa, Canada).

    Article Title: CF2H: a Cell-Free Two-Hybrid platform for rapid protein binder screening
    Article Snippet: Cells were lysed using an Avestin EmulsiFlex-C3 homogenizer at 15000-20000 psi, and the supernatant was dialysed overnight in S30B.

    Article Title: Structural determinants for pH-dependent activation of a plant metacaspase.
    Article Snippet: Cells were lysed by using an EmulsiFlex-C3 Homogenizer (Avestin, Ottawa, Canada), followed by the addition of Triton X-100 (Sigma) to a final concentration of 1% and stirring at 4 °C for 1 h. Subsequently, the lysates were centrifuged at 18,000 × g for 1 h, and the supernatant was subjected to purification using Glutathione Sepharose 4B resin and a PD10 column, following the manufacturer’s protocols (GE Healthcare, Inc.).

    Article Title: Cation-Driven Modulation of Tau Condensates: Insights into Liquid-Liquid Phase Separation and Rheological Properties.
    Article Snippet: The formation of biocondensates through liquid−liquid phase separation (LLPS) has emerged as a vital and ubiquitous phenomenon contributing to the spatiotemporal coordination of cellular processes.. Additionally, dysregulation of LLPS is increasingly implicated as a previously overlooked driver of diseases.. LLPS typically involves multivalent noncovalent interactions among biomolecules, yet the role of solvent molecules, particularly water, in this process has received increasing attention.

    Article Title: Allosteric regulation of L‐lactate dehydrogenase: Beyond effector‐mediated tetramerization
    Article Snippet: Following overnight growth (typical final turbidity of OD 600 12) the cells were harvested, resuspended in 45 mL of buffer (20 mM Tris–HCl pH 7.5, 500 mM NaCl, 2.5% glycerol, 0.5 mM CaCl 2 , and 20 mM imidazole), disrupted by two passages through an EmulsiFlex‐C3 homogenizer (Avestin, Inc., Ottawa, Canada), and centrifuged (16,000 g for 20 min) to obtain a soluble extract.

    Article Title: Modularity of Zorya defense systems during phage inhibition
    Article Snippet: Briefly, cells were grown at 37 °C for 15 h in a terrific broth medium containing kanamycin (50 μg/mL) and l -Rhamnose monohydrate (0.1% w / v ) and then collected by centrifugation at 4000× g . Cell pellets were resuspended in Tris-buffered saline (TBS) (100 mM of Tris, 150 mM of NaCl, 1 mM of EDTA, pH 8.0) plus 30 μg/mL of DNase I and 400 μg/mL of lysozyme for 30 min before passage through an EmulsiFlex-C3 homogenizer (Avestin) at 15,000 psi.

    Article Title: Allosteric regulation of L-lactate dehydrogenase: Beyond effector-mediated tetramerization.
    Article Snippet: Following overnight growth (typical final turbidity of OD600 12) the cells were harvested, resuspended in 45 mL of buffer (20 mM Tris–HCl pH 7.5, 500 mM NaCl, 2.5% glycerol, 0.5 mM CaCl2, and 20 mM imidazole), disrupted by two passages through an EmulsiFlex-C3 homogenizer (Avestin, Inc., Ottawa, Canada), and centrifuged (16,000 g for 20 min) to obtain a soluble extract.

    Purification:

    Article Title: Application of Amber Suppression To Study the Role of Tyr M210 in Electron Transfer in Rhodobacter sphaeroides Photosynthetic Reaction Centers.
    Article Snippet: In each round, the cell slurry was passed more than three times through an Avestin EmulsiFlex-C3 Homogenizer operating at 20,000 – 25,000 psi.

    Article Title: Structural determinants for pH-dependent activation of a plant metacaspase.
    Article Snippet: Cells were lysed by using an EmulsiFlex-C3 Homogenizer (Avestin, Ottawa, Canada).

    Article Title: CF2H: a Cell-Free Two-Hybrid platform for rapid protein binder screening
    Article Snippet: Cells were lysed using an Avestin EmulsiFlex-C3 homogenizer at 15000-20000 psi, and the supernatant was dialysed overnight in S30B.

    Article Title: Structural determinants for pH-dependent activation of a plant metacaspase.
    Article Snippet: Cells were lysed by using an EmulsiFlex-C3 Homogenizer (Avestin, Ottawa, Canada), followed by the addition of Triton X-100 (Sigma) to a final concentration of 1% and stirring at 4 °C for 1 h. Subsequently, the lysates were centrifuged at 18,000 × g for 1 h, and the supernatant was subjected to purification using Glutathione Sepharose 4B resin and a PD10 column, following the manufacturer’s protocols (GE Healthcare, Inc.).

    Article Title: Cation-Driven Modulation of Tau Condensates: Insights into Liquid-Liquid Phase Separation and Rheological Properties.
    Article Snippet: The formation of biocondensates through liquid−liquid phase separation (LLPS) has emerged as a vital and ubiquitous phenomenon contributing to the spatiotemporal coordination of cellular processes.. Additionally, dysregulation of LLPS is increasingly implicated as a previously overlooked driver of diseases.. LLPS typically involves multivalent noncovalent interactions among biomolecules, yet the role of solvent molecules, particularly water, in this process has received increasing attention.

    Article Title: Allosteric regulation of L‐lactate dehydrogenase: Beyond effector‐mediated tetramerization
    Article Snippet: Following overnight growth (typical final turbidity of OD 600 12) the cells were harvested, resuspended in 45 mL of buffer (20 mM Tris–HCl pH 7.5, 500 mM NaCl, 2.5% glycerol, 0.5 mM CaCl 2 , and 20 mM imidazole), disrupted by two passages through an EmulsiFlex‐C3 homogenizer (Avestin, Inc., Ottawa, Canada), and centrifuged (16,000 g for 20 min) to obtain a soluble extract.

    Article Title: Modularity of Zorya defense systems during phage inhibition
    Article Snippet: Briefly, cells were grown at 37 °C for 15 h in a terrific broth medium containing kanamycin (50 μg/mL) and l -Rhamnose monohydrate (0.1% w / v ) and then collected by centrifugation at 4000× g . Cell pellets were resuspended in Tris-buffered saline (TBS) (100 mM of Tris, 150 mM of NaCl, 1 mM of EDTA, pH 8.0) plus 30 μg/mL of DNase I and 400 μg/mL of lysozyme for 30 min before passage through an EmulsiFlex-C3 homogenizer (Avestin) at 15,000 psi.

    Article Title: Allosteric regulation of L-lactate dehydrogenase: Beyond effector-mediated tetramerization.
    Article Snippet: Following overnight growth (typical final turbidity of OD600 12) the cells were harvested, resuspended in 45 mL of buffer (20 mM Tris–HCl pH 7.5, 500 mM NaCl, 2.5% glycerol, 0.5 mM CaCl2, and 20 mM imidazole), disrupted by two passages through an EmulsiFlex-C3 homogenizer (Avestin, Inc., Ottawa, Canada), and centrifuged (16,000 g for 20 min) to obtain a soluble extract.

    Centrifugation:

    Article Title: Application of Amber Suppression To Study the Role of Tyr M210 in Electron Transfer in Rhodobacter sphaeroides Photosynthetic Reaction Centers.
    Article Snippet: In each round, the cell slurry was passed more than three times through an Avestin EmulsiFlex-C3 Homogenizer operating at 20,000 – 25,000 psi.

    Article Title: Structural determinants for pH-dependent activation of a plant metacaspase.
    Article Snippet: Cells were lysed by using an EmulsiFlex-C3 Homogenizer (Avestin, Ottawa, Canada).

    Article Title: CF2H: a Cell-Free Two-Hybrid platform for rapid protein binder screening
    Article Snippet: Cells were lysed using an Avestin EmulsiFlex-C3 homogenizer at 15000-20000 psi, and the supernatant was dialysed overnight in S30B.

    Article Title: Structural determinants for pH-dependent activation of a plant metacaspase.
    Article Snippet: Cells were lysed by using an EmulsiFlex-C3 Homogenizer (Avestin, Ottawa, Canada), followed by the addition of Triton X-100 (Sigma) to a final concentration of 1% and stirring at 4 °C for 1 h. Subsequently, the lysates were centrifuged at 18,000 × g for 1 h, and the supernatant was subjected to purification using Glutathione Sepharose 4B resin and a PD10 column, following the manufacturer’s protocols (GE Healthcare, Inc.).

    Article Title: Cation-Driven Modulation of Tau Condensates: Insights into Liquid-Liquid Phase Separation and Rheological Properties.
    Article Snippet: The formation of biocondensates through liquid−liquid phase separation (LLPS) has emerged as a vital and ubiquitous phenomenon contributing to the spatiotemporal coordination of cellular processes.. Additionally, dysregulation of LLPS is increasingly implicated as a previously overlooked driver of diseases.. LLPS typically involves multivalent noncovalent interactions among biomolecules, yet the role of solvent molecules, particularly water, in this process has received increasing attention.

    Article Title: Allosteric regulation of L‐lactate dehydrogenase: Beyond effector‐mediated tetramerization
    Article Snippet: Following overnight growth (typical final turbidity of OD 600 12) the cells were harvested, resuspended in 45 mL of buffer (20 mM Tris–HCl pH 7.5, 500 mM NaCl, 2.5% glycerol, 0.5 mM CaCl 2 , and 20 mM imidazole), disrupted by two passages through an EmulsiFlex‐C3 homogenizer (Avestin, Inc., Ottawa, Canada), and centrifuged (16,000 g for 20 min) to obtain a soluble extract.

    Article Title: Modularity of Zorya defense systems during phage inhibition
    Article Snippet: Briefly, cells were grown at 37 °C for 15 h in a terrific broth medium containing kanamycin (50 μg/mL) and l -Rhamnose monohydrate (0.1% w / v ) and then collected by centrifugation at 4000× g . Cell pellets were resuspended in Tris-buffered saline (TBS) (100 mM of Tris, 150 mM of NaCl, 1 mM of EDTA, pH 8.0) plus 30 μg/mL of DNase I and 400 μg/mL of lysozyme for 30 min before passage through an EmulsiFlex-C3 homogenizer (Avestin) at 15,000 psi.

    Article Title: Allosteric regulation of L-lactate dehydrogenase: Beyond effector-mediated tetramerization.
    Article Snippet: Following overnight growth (typical final turbidity of OD600 12) the cells were harvested, resuspended in 45 mL of buffer (20 mM Tris–HCl pH 7.5, 500 mM NaCl, 2.5% glycerol, 0.5 mM CaCl2, and 20 mM imidazole), disrupted by two passages through an EmulsiFlex-C3 homogenizer (Avestin, Inc., Ottawa, Canada), and centrifuged (16,000 g for 20 min) to obtain a soluble extract.

    Saline:

    Article Title: Application of Amber Suppression To Study the Role of Tyr M210 in Electron Transfer in Rhodobacter sphaeroides Photosynthetic Reaction Centers.
    Article Snippet: In each round, the cell slurry was passed more than three times through an Avestin EmulsiFlex-C3 Homogenizer operating at 20,000 – 25,000 psi.

    Article Title: Structural determinants for pH-dependent activation of a plant metacaspase.
    Article Snippet: Cells were lysed by using an EmulsiFlex-C3 Homogenizer (Avestin, Ottawa, Canada).

    Article Title: CF2H: a Cell-Free Two-Hybrid platform for rapid protein binder screening
    Article Snippet: Cells were lysed using an Avestin EmulsiFlex-C3 homogenizer at 15000-20000 psi, and the supernatant was dialysed overnight in S30B.

    Article Title: Structural determinants for pH-dependent activation of a plant metacaspase.
    Article Snippet: Cells were lysed by using an EmulsiFlex-C3 Homogenizer (Avestin, Ottawa, Canada), followed by the addition of Triton X-100 (Sigma) to a final concentration of 1% and stirring at 4 °C for 1 h. Subsequently, the lysates were centrifuged at 18,000 × g for 1 h, and the supernatant was subjected to purification using Glutathione Sepharose 4B resin and a PD10 column, following the manufacturer’s protocols (GE Healthcare, Inc.).

    Article Title: Cation-Driven Modulation of Tau Condensates: Insights into Liquid-Liquid Phase Separation and Rheological Properties.
    Article Snippet: The formation of biocondensates through liquid−liquid phase separation (LLPS) has emerged as a vital and ubiquitous phenomenon contributing to the spatiotemporal coordination of cellular processes.. Additionally, dysregulation of LLPS is increasingly implicated as a previously overlooked driver of diseases.. LLPS typically involves multivalent noncovalent interactions among biomolecules, yet the role of solvent molecules, particularly water, in this process has received increasing attention.

    Article Title: Allosteric regulation of L‐lactate dehydrogenase: Beyond effector‐mediated tetramerization
    Article Snippet: Following overnight growth (typical final turbidity of OD 600 12) the cells were harvested, resuspended in 45 mL of buffer (20 mM Tris–HCl pH 7.5, 500 mM NaCl, 2.5% glycerol, 0.5 mM CaCl 2 , and 20 mM imidazole), disrupted by two passages through an EmulsiFlex‐C3 homogenizer (Avestin, Inc., Ottawa, Canada), and centrifuged (16,000 g for 20 min) to obtain a soluble extract.

    Article Title: Modularity of Zorya defense systems during phage inhibition
    Article Snippet: Briefly, cells were grown at 37 °C for 15 h in a terrific broth medium containing kanamycin (50 μg/mL) and l -Rhamnose monohydrate (0.1% w / v ) and then collected by centrifugation at 4000× g . Cell pellets were resuspended in Tris-buffered saline (TBS) (100 mM of Tris, 150 mM of NaCl, 1 mM of EDTA, pH 8.0) plus 30 μg/mL of DNase I and 400 μg/mL of lysozyme for 30 min before passage through an EmulsiFlex-C3 homogenizer (Avestin) at 15,000 psi.

    Article Title: Allosteric regulation of L-lactate dehydrogenase: Beyond effector-mediated tetramerization.
    Article Snippet: Following overnight growth (typical final turbidity of OD600 12) the cells were harvested, resuspended in 45 mL of buffer (20 mM Tris–HCl pH 7.5, 500 mM NaCl, 2.5% glycerol, 0.5 mM CaCl2, and 20 mM imidazole), disrupted by two passages through an EmulsiFlex-C3 homogenizer (Avestin, Inc., Ottawa, Canada), and centrifuged (16,000 g for 20 min) to obtain a soluble extract.



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